Fig. 3. NO levels of HCAECs treated with TNFα, TNFα and EMPA or DAPA. Cells were treated with 0.02% DMSO (control), 1 µM EMPA or DAPA, 10 ng/mL TNFα or 10 ng/mL TNFα with 1 µM EMPA or DAPA for 6h. NO levels were measured using live cell imaging in HCAECs (a,b n=4) Representative images are shown in the panels below each figure. Data are presented as mean±SD. *p<0.05 vs. TNFα, **p<0.01, ***p<0.001 vs TNFα.